The In-vivo Experimental Investigation Of Prolonged Preservation In Rat Heart With Low Flow Perfusion And Coronary Oxygen Persufflation Techniques

Part One The establishment and comparison of two type of heterotopic heart transplantation model in rat【Abstract】Objective To establish and evaluate the improved Heron and Ono model of rat heart transplantation. Methods We choose the SD rat, 30 as the donor and 30 as the recipient to establish 15 Heron models and 15 Ono models. Results 100% of Heron models and 86.7% of Ono models were successful established. In the Heron model, the time of the full operation and the anastomosis was significantly shorter than that of Ono model(P<0.01).The postop. survival rate of the Ono model is significantly higher than that of Heron model (P<0.01). Conclusions The procedure of the Heron model has shorter operation time and better protection of donor heart and is applicable to the following investigation.Part Two The role of low flow perfusion and coronary oxygen persufflation techniques in the prolonged rat heart preservation【Abstract】Objective To explore the change of structure and function of the rat donor heart after the preservation of low flow perfusion and coronary oxygen persufflation techniques. And to discover the applicability of LFP and COP in clinical heart transplantation. Methods 60 SD rats were chosen to be the donors and recipients and was divided into 3 groups at random. The donor heart was infused by 4℃St.ThomasⅡs olution and was preserved in 4℃NS. Group NS, donor heart was simply preserved in 4℃NS; Group LFP,donor heart was continuously infused by HTK solution via the ascending aorta at the speed of 5ml/g heart/24h. Group COP, donor heart was persufflated by pure oxygen via the ascending aorta at the pressure of 25cm H2O.All the three groups were reinfused by HTK solution at the speed of...