| Objective: Disorders of cortical development (DCDs) are a frequent cause of intractable epilepsy in humans. While the pathogenesy of DCDs is still poorly understood. To study the mechanism underlying DCDs, we developed two experimental models of DCDs to study their histopathologic characteristics and genes related to such characteristics.Methods: Time-pregnant Sprague-Dawley rats were irradiated on embryonic day 15 (E15) with 1.45 gray (Gy), or were given intraperitoneal injection with 1-3-bis-chloroethyl-nitrosourea (BCNU), or were left untreated. One pregnant rat of each group was allowed to raise its litters. The litters were killed at postnatal day 30 (P30) and the brains were processed for histopathologic studies. Here we labelled Cajal-Retzius (CR) cells with anti-calretinin to study the abnormal existence of these cells in the brains of our animal models of DCDs. Other litters were killed in 12 hours after delivery, the brains of each group were taken for studing related genes by using Affymetrix genechips. The total RNAs were isolated from...
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