Clinical Sigificance Of Peripheral Blood Detection Of Micrometsastasis In Patients With Primary Hepatocellular Carcinma

Objective: To establish the molecular method to detect peripheral blood micrometsastasis in patients with Primary hepatocellular carcinoma(PHC),and discuss its clinical sigificance.Methods: A sensitive RT-PCR system was set up to detect AFPmRNA and CD44v6mRNA in the peripheral blood of 50 PHC patients, 10 hepatic hemangioma patients ,10 hepatic cyst patients,10 hepatocirrhosis patients,10 chronic hepatitis patients, 20 patients with metastatic liver cancer, and 30 healthy volunteers.Results: Of the PHC patients, expression of AFPmRNA and CD44v6mRNA was positive respectively in 50%(25/60) and 58%(29/50) of the peripheral bloods.while 37(74% )PHC patients were positive for at least 1 marker. With regard to controls, 3 of 20 samples(15%)from group hepatitis and cirrhosis showed AFPmRNA,but none of samples from other control group showed AFPmRNA. And 9 of 20(45%)samples from patients with metastatic liver cancer showed CD44v6mRNA in their peripheral blood,but groups of hepatitis and cirhosis,hepatic hemangioma,hepatic...