A Study On The Differentiation From Neonatal Rat Neural Stem Cells To Oligodendrocytes

PART ONE ISOLATION AND CULTIVATION OF NEONATAL RAT NEURAL STEM CELLSBackground and Objective Neural stem cell, the self-renewing multipotential cell, can generate neural tissue, including neurons, astrocytes and oligodendrocytes. It exist not only in the developing mammalian nervous system but also in the adult nervous system of all mammalian organisms, including human. Isolation and culture of NSC is the basement of the fundamental and applied research. Objective of this part is to obtain neonatal rat NSCs for further study on its proliferation and differentiation.Methods Isolation the primary NSCs from endbrain and midbrain of neonatal rats (in 24h after birth), the cells are suspended and cultured in serum free medium (containing bFGF and EGF) for long-term survival in vitro. Technique of single-cell clone culture was used to confirm the character of self-renewing. Ability of multi-directional differentiation and...