Expression, Purification Of The MMP9-PEX In E.Coil And Its Effect On Invasive Capacity Of Colonic Cancer Cells

Almost all MMPs share a homologous C-terminal haemopexin-like domain(PEX). Many differrent proteins can bind with high affinity to MMPs at the PEX domain. The function of MMPs will be inhabited when the PEX domain appears to mutate. A 29kDa-32kDa C-terminal fragment comprises the hemopexin domain, which derived from the autocatalytic digestion of MMPs. PEX is detected in the cultured medium of various human glioma, endothelial, breast, and prostate carcinoma cell lines. PEX fragment which inhibits angiogenesis in the different pathological and physiological conditions is an endogenous inhibitor of MMPs.During the process of the tumour migration and embryonic implantanion, MMPs play important roles, especially MMP2 and MMP9. They can promote tumor cell and trophoblast cell invasion through degrading ECM.Therefore, as an endogenous inhibitor of MMPs, PEX may have important function in these process. However, its function in embryonic implantation was further characterized.