The Study Of Long-term Islet Graft Survival In Islet Transplant Diabetic Mice Treated With Tacrolimus, Rapamycin, And Anti-interleukin-2 Antibody

BackgroundBackground: Islet transplantation is a treatment for patients with type I diabetes mellitus with substantial advantages . To assesee the preventive effects of tacrolimus,rapamycin and Anti IL-2 antibody on islet graft acute rejection in rat-to-mouse model. C57BL/6 (B6) mice were used as recipients and Wistar mice as donors.The islets of rat were transplanted under the kidney capsule of diabetic mouse.The recipients were randomly divided into control group, tacrolimus+rapamycin group, tacrolimus+ Anti IL-2 antibodygroup, rapamycin+ Anti EL-2 antibody group, tacrolimus+rapamycin+ Anti IL-2 antibody group. The concurrence of allorecognition and recurrence of autoimmunity might explain why most of the treatments successful in preventing islet allograft destruction in other nonautoimmune combinations often fail in C57BL/6 (B6) recipients. To assess the value of the C57BL/6 (B6 ) mouse model for the evaluation of treatments relevant to clinical islet transplantation, tested the effect of a protocol closely resembling the one successfully used in the Edmonton clinical trial on the survival of islet allografts in C57BL/6 (B6) mice.Methods:Murine islets were isolated and purify from Wistar mice. The pancreatic islets were isolated by collagenase digestion and then purified by Ficoll density...