Study On The Method Of Qulity Control For Hedyotis Diffusa Willd. And Its Injection

Hedyotis diffusa Willd. is a kind of folk medicine, possessing wide pharmacological activities. With the development of modern pharmacology study and clinic applications of its immunity and other activities, Hedyotis diffusa Willd. increasingly attracts the attention of pharmaceutical researchers all over the world. The purpose of this thesis is to develop methods for the quality control of Hedyotis diffusa Willd. and its injection. An active constituent, E-6-O-p-coumaroyl scandoside methyl ester, was isolated from Hedyotis diffusa Willd. and identified by using UV, MS, ¹H-NMR and ¹³C-NMR. High performance liquid chromatography fingerprint spectrum (HPLC-FPS) were developed for identification of Hedyotis diffusa Willd., the injection and its intermediate. The contents of oleanolic acid and ursolic acid in raw materials of Hedyotis diffusa Willd. from different sources were simultaneously determined. HPLC methods for determination of p-coumaric acid in Hedyotis diffusa Willd. and its injection were developed. 1. The preparation of E-6-O-p-coumaroyl scandoside methyl esterIt is reported that there are a few kinds of active substances presenting in Hedyotis diffusa Willd. such as anthraquinones, iridoids, triterpeniods and coumarins. E-6-O-p-coumaroyl scandoside methyl ester is one of iridoids...