| Objective To study the effect of the repair of rabbit articular cartilage defects by the composite of chondrogenic induction of autologous marrow mesenchymal stem cells(MSCs) and autologous "two-phase" bone matrix gelatin(BMG). Methods 1. The bone marrow which was aspirated from the rabbit's shinbone,high glucose Dulbecos modified Eagle medium(H-DMEM) was added into the marrow, after blowing, beating, and centrifuging, cells suspension was inoculated in the culture bottle to preceed the primary culture, which changed the culture medium gradually to take away cells which didn't stick the wall to abtain the MSCS.2. It was easy to get enough quantity of the MSCs through transferring in vitro, observed its morphology, drew the growth curve.3. When the MSCs gathered together,the growth factor: transforming growth factor-betal (TGF- β1), insulin-like growth factor-I (IGF-I ),vitamin C were added in and induced the MSCs to convert to cartilage cells, and provided the seed cells for repairing the cartilage defects in tissue engineering.4. Getting out the part of the rabbit s ilium, according to the method of modified Urist, the "two-phase" BMG was acquired from degreasing, taking off the calcium and getting out the protein, and was cut as the certain size and shape as needed. It could be conserved after sterilizing within a low temperature.5. Inoculated chondrogenic induction of autologous MSCs into autologous BMG to set up a composite of the cell-carrier, observed it through the electric-microscope after culturing three days.6. Made the model of articular cartilage defects of rabbits, divided it into three groups, the experimental group was implanted the composite, the experimental...
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