Study Of Treating Human Primary Hepatocellular Carcinoma By AFP/ANGIO/TK Targeted Fusion Gene

Objective: To study the treating effective of nude mouse model transplanted with human liver cancer cell strain SMMC-7721 by pcDNA3/AFP/angio/tk targeted fusion gene. Method: To construct nude mouse sub-skin model transplanted with human primary liver cancer. When the diameter of the tumor got to 4～5mm, the nude mice were divided randomly into five groups: control group; vector group; GCV group; pcDNA3/angio/tk group; pcDNA3/AFP/angio/tk group. Different plasmas were directly injected into tumor and GCV intraperitoneal injection was given simultaneously according to different groups. Observing the tumor growth condition and calculating the volume of tumor; pathologic examination; detecting cell apoptosis in suit by TUNEL method and electron microscope. Results: The results of pathologic examination and detecting cell apoptosis in suit by TUNEL method indicated that pcDNA3/AFP/angio/tk group could remarkably inhibit the growth of tumor and was more effective than pcDNA3/angio/tk group(P<0.05). Conclusion: pcDNA3/AFP/angio/tk targeted fusion gene system would remarkably inhibit the growth of tumor, and the treating effects to human liver cancer by fusion gene AFP/angio/tk were more efficient than those by angio/tk fusion gene.