| OBJECTIVES: To observe the effects of Class III orthopedic forces in the sutures around the maxillary, by investigating these sutures, and to visit the mechanism of Class III malocclusion treatment.SUBJECTS AND METHODS: 6 healthy male Rhesus monkeys, which had mixed dentition, were employed in this study. These monkeys were divided into 3 groups, 4 monkeys were used as one and a half months and three months experimental groups, 2 monkeys served as control. Each of the experimental monkeys were fitted with casted Twin-block Magnetic Appliance III (TMA-III). For evaluating the effects of protraction of dentofacial complex, oral pictures, model casts and lateral cephlograms of each animal were taken at the beginning, during and at the end of experiment. Before taking the cephalograms, mental paired implants of silver amalgam were placed across the zygmaticomaxilary suture, zygmaticotemprol suture and zygmaticofrontal suture in each animal. Separating the sutures around the maxillary, decalcificating and embedding these samples, we observe the change of the sutures by HE staining, Masson staining and electron microscope. We detect the expression of PCNA, collagen I and collagen III in these sutures.RESULTS: Through measurements of the sutures of experimental animals, we find that the width of sutures are increased. ; by HE staining and Masson staining, we find that both osteoblasts and fibroblasts are increased;... |