| Objective: To compare the distribution and cellular localization of BDNF and NT-3 in central nervous system of adult brains of rats and monkeys by both immunohistochemistry and Western blotting, and also give primary discussion about the temporal changes and endogenous action mechanizims of BDNF and NT-3 following hemisected spinal cord injury.Method: In the first part of the research, Adult male Sprague-Dawley rats (200g～250g) and youth male Rhesus monkeys weighing about 7kg were used in our study. After perfusion, the different parts of the brains were removed. Samples from rats and monkeys were processed for neurotrophin immunohistochemistry simultaneously. And then, fresh brain samples from rats and monkeys were obtained for the use of semi-qualification by Western blotting. In the second part of the research, 21 adult Rhesus were divided into seven groups randomly. Spinal cord hemisection were performed at T11 level in rhesus from five groups, while control group did not receive half-transection of the left spinal cord. Hindlimb function was evaluated and cortical somatosensary evoked potentials(CSEP) were recorded after operation. The aminals received spinal cord hemisection were allowed to survive for 1days, 7 days, 14days, 30days, 60days and 90days. The caudal and rostral 1 cm spinal cord around the injury site were obtained, and 20 μm thick frozen sections were cut. By the immunohistochemical SP method, we observed the distribution of BDNF and...
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