Research On The Relationships Between Abnormal CD95 And CD137-mediated Signaling Pathways Of T Lymphocyte Subset And Disturbance Of Lymphocyte Apoptosis In Systemic Lupus Erythematosus

Objective To analyze apoptosis state of T lymphocytes in patients with Systemic Lupus Erythematosus (SLE), the roles of CD95 and CD137 mediated signaling pathways in apoptosis of T lymphocyte subsets, as well as the relationships of anti-nucleosome antibodies and disturbance of apoptosis regulation in order to discuss disturbance of T lymphocyte apoptosis and the abnormal apoptosis signaling pathways, further to lay the groundwork for finding key targets of abnormal signaling pathways in T lymphocytes in patients with SLE.Methods Samples of 39 patients with SLE (including 22 patients with inactive SLE and 17 patients with active SLE) and 13 health volunteers were analyzed to learn apoptosis state of peripheral blood lymphocytes. Flow cytometry and AnnexinV-FITC/PI double dyeing were applied to determine the percentages of apoptotic lymphocytes and necrotic lymphocytes. Flow cytometry and fluorescein-labeled monoclone antibodies were applied to determine the expression rates of membrane CD95,CD95L,CD137 and...