The Study Of The Anti-leukemia Effect Of The IFNγ Gene Transfered MSCs In Vitro

Objective:1. To observe the growth characteristic of the Mesenchymal stem cells (MSCs) in vitro and to make the recombinant adenovirus vectors ( AdGFPand AdIFNγ) for transduction.2. To investigate the effects of the adenovirus transduction on the growth of MSCs and on the expression of purpose gene; To explore the appropriate condition of the adenovirus vectors transfered to MSCs.3. To investigate the anti-leukemia effects of the IFN7 gene transfered MSCs on the K562 cells in vitro.Methods:1. In vitro,the frozen MSCs which were stored in liquid nitrogen was thawed,cultivated and expanded by L-DMEM cultivate medium which contained 10% FBS.Everyday MSCs were harvested with trypsin and counted.By this way the growth curve of MSCs could be figured out;The rAd vectors (AdGFP and AdIFNγ) were largely expanded and produced by 293...