The Resistance To Carbapenems And The Correlation Between Resistance Phenotype And Expression Levels Of Efflux Pumps Of Pseudomonas Aeruginosa

Objective: To analyse the resistance profile of 142 strains of Pseudomonas aeruginosa(Pa) isolated from our hospital to 8 antimicrobial agents; to investigate the effects of efflux pump inhibitors (CCCP and PAβN) to carbapenems in Pa clinical isolates; To investigate the correlation between the resistance phenotypes and expression levels of efflux pumps and the amounts of OpD2 of Pa ,to discuss the mechanism of different resistant phenotypes in Pa, and to confirm the role of efflux pumps and OpD2 in the resistance to imipenem and meropenem in clinical strains of Pa.Methods: The minimal inhibitory concentrations (MICs) of 8 antimicrobial agents comprising imipenem, meropenem, ceftazidime, piperacillin/tazobactam, cefoperazone/sulbactam, aztreonam, ciprofloxacin and amikacin against 142 clinical isolates were determined by agar dilution method according to NCCLS. MICs of imipenem or meropenem combined with efflux pump inhibitors against 107 imipenem resistant strains were...