| In this study, we used the terminal differentiation model of human osteosarcoma MG-63 cells built by our research group to investigate the induction effects of ginsenoside Rg1, cinnamic acid , tanshinoneâ…¡A and ginsenoside Rg1 + cinnamic acid, ginsenoside Rg1 + cinnamic acid + tanshinoneâ…¡A on the terminal differentiation of human osteosarcoma MG-63 cells. The aim is to further identify the induction effects of effective ingredients of Chinese traditional medicine on terminal differentiation of tumour cells and explore the machamism of terminal differentiation induction primarily.Our results revealed that the proliferation of MG-63 cells was inhibited differently after the treatments of ginsenoside Rg1, cinnamic acid, tanshinoneâ…¡A and their combinations. The inhibitory rates were 22.06%,54.94%,52.10%,59.23% and 72.37% and the doubling time was prolonged from 47.96h to 52.69h,67.29h,65.27h,70.88h and 89.39h. Flow cytometry analysis showed that MG-63 cells were arrested in G0/G1 phase after the treatments. The proportion of cells in G0/G1 phase rose from 47.5% to 56.6%,59.8%,59.5%,67.9% and 72.5% while the proportion of cells in S phase declined from 20.0% to 11.8%,10.3%,9.0%,8.2% and 7.3%.With the light microscope and transmission electron microscope, we observed that the MG-63 cells had undergone malignant morphology and ultrastructure reversion after the treatments. The morphology of the cells was regular and the size of them tended to be similar. The size of the cells increased. And the proportion of nucleus to cytoplasm decreased. The morphology of the nucleus was regular. The heterochromatin decreased while the euchromatin increased. The number of mitochondrions increased and their size appeared similar. Rough endoplasmic reticulum and free ribosomes increased while polyribosomes decreased. The...
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