The Expression Of AEs Protein In The Anoxia/reoxygenation Injury Of Cardiac Myocytes

Objective: Anion exchange proteins, which facilitate the reversible electroneutral exchange of Cl^- for HCO₃^- across the plasma membrane, regulate pHj, intracellular chloride concentration, bicarbonate metabolism and cell volume. The AE family comprises three members: AE1, AE2, and AE3. Our study was to explore the expression of AEs proteins in the anoxia/reoxygenation injury of neonatal cardiac myocytes.Methods: RT-PCR and Western blotting were used to measure the different AE isoform mRNA and protein expression in primary neonatal cardiac myocytes A/R model and APC model respectively. Cellular injury was evaluated by measuring cardiac myocyte beating rate, cell viability, and lactate dehydrogenase (LDH) release. The cardiac myocytes beating rates were counted under inverted microscope at the end of experiment. Cell viability was determined after treatment using MTT assay. The activity of LDH in culture medium was measured using an automatic biochemical analyzer. Cultured cardiac myocytes of neonatal SD rats have been randomly divided into four groups: CD control group, cells were incubated under normoxic conditions. (2)AJ R group: 3 h anoxia was followed by reoxygenation for 2 h. (3) anoxia preconditioning group-early phase (APC-EP), 3 cycles of 30 min anoxia and 30 min reoxygenation ,consecutively, cardiac myocytes was subjected to A/R injury.(4)anoxia preconditioning group-delayed phase (APC-DP), anoxia preconditioning was followed by 24 h normoxic incubation prior to A/ R.Results: In A/R group, cardiac myocyte beating rate was significantly slower, and the cell viability was noticeably decreased, additionally, LDH activity was...