Inhibitory Effects Of Panaxydol On The Proliferation And Migration Of Vascular Smooth Muscle Cells And Its Mechanism

AIM: The inhibitory effects of panaxydol on the proliferation and migration of VSMCs (vascular smooth muscle cells) induced by 10%FBS and its mechanism were investigated.METHODS: 3～8 passages of primary cultured rat aortic smooth muscle cells (RASMCs) were used. RASMCs were grouped as follows: ①control(contained 0.1%DMSO); ②10%FBS③l, 3, 9μmol·L^-1 panaxydol (added 10%FBS after a 30-min incubation of panaxydol ). The effects of different concentrations of panaxydol on the proliferation and migration of RASMCs were evaluated by MTT and cell scrape tests. The content of hydroxyproline was observed by Colorimetry method. The influences of panaxydol on the expression of mtTF1 mRNA were studied by RT-PCR.RESULTS: MTT tests showed that 10%FBS could increase A values significantly compared with control. 1, 3, 9μmol·L^-1 panaxydol pretreatment decreased A values obviously and inhibitory rates were 11.7%(p<0.05～0.01), 21.9%(p<0.01) and 30.0%(p<0.01) respectively. The results suggested that panaxydol could inhibit RASMCs hyperplasia induced by 10%FBS concentration-dependently. Cell scrape tests showed that 10%FBS could increase the distance of migration in RASMCs compared with control(p< 0.01). 1, 3, 9μmol·L^-1 panaxydol counteracted the action of 10%FBS, inhibitory rates of migration were 4.4%(p< 0.05～0.01), 12.5%(p<0.01) and 18.5%(p<0.01) respectively, which indicated that panaxydol could inhibit the RASMCs migration induced by 10%FBS. In addition, colorimetry method results showed that 1, 3, 9μmol·L^-1 panaxydol decreased the A values significantly,inhibitory rates of hydroxyproline synthesis were 2.8%(p<0.05～ 0.01), 11.8%(p<0.01) and 27.9%(p<0.01) respectively, which indicated that...