Effect Of Microencapsulated Olfactory Bulb Xenotransplantation On Cells Apoptosis And Functional Recovery After Spinal Cord Injury In Rats

Objective To observe the effect of microencapsulated rabbit olfactory bulb cells xenotransplantation on cells apoptosis and functional recovery after spinal cord transection in rats.To explore its protective mechanism during the period of secondary affection. The aim of this study was to demonstrate that the enclosured olfactory bulb cells in a selective permeable membrane should be designed to realize allo-and xeno-transplantation without immunosuppressive therapy. This results may serve as an important step toward the clinical application for spinal cord injury patients. Methods Transected injury at the T₁₀ level of the spinal cord was performed on Sprage Dawley (SD) rats and the microcapsules, which were absorbed into gelfoams(absorbable gelatin sponge), were grafted into the lesion site. All the rats were randomly divided into 4 groups: A-normal control group; B-injury control group; C-cell grafted group and D-microcapsule grafted group(rats were transplanted with gelfoams which have absorbed microcapsulated rabbit olfactory bulb cells) .At 12 hours and 1,3,7,21 days after injury , a 10mm cord segment centered at the lesion site was removed , was placed in the same fixative overnight, and was embedded in paraffin. Serial 5um crosssections were cut and stained with the methods of the terminal deoxynucleotidal transferase-mediated DUTP-biotinnick end labeling (TUNEL),HE and Nissl , before each rat was sacrificed, BBB scale was designed to assess it's behavioural ability. Results Few TUNEL-posi-tive cells were noted in A group;TUNEL method showed that B group had a large number of apoptosis cells. The statistics showed that there...