The Cloning And Transient Transfection Of Human Antimicrobial Peptide HCAP-18/LL-37 And The Effect On The Growth Of MCF-7 Cells

Malignant tumor is one of the major diseases that threaten human health. It is urgent for people to find and screen more effective anti-tumor medicines. In addition to the broad-spectrum activity against bacteria, fungi, parasites, and viruses, cationic antimicrobial peptides, components of the innate host defenses, also have a fairly anti-tumor activity. The peculiar mechanisms of their anti-tumor and the very low or no toxicity to normal cells have made them on the focus of attention to develop a novel anti-tumor medicine. In this study, we constructed the recombinant of hCAP-18/LL-37, the unique known antimicrobial peptide of Cathelicidins family that exits in human body, using molecular biological techniques; then infected MCF-7 cells by way of transient transfection, aiming at studying the role of hCAP-18/LL-37 in the activity of anti-tumor.Objective: In this study, we set up to construct the recombinant of eukaryotic expression of hCAP-18/LL-37, and transfer it into human breast tumor cell strain MCF-7 to explore the mechanism of its anti-tumor activity.Methods: (1) The cDNA containing the complete sequence of hCAP-18/LL-37 was obtained by PCR technique using the recombinant pCDNA4.0-LL-37. (2) PMD18-T-LL-37, the...