Study On The Improved Model And Neuroprotection Of GM1 On Middle Cerebral Artery Occlusion Reperfusion In Rats

Part 1.The Study on the Improved Model of Middle Cerebral Artery Occlusion ReperfusionObjective: To evaluate the modified method of suture-embolus by which to make the model of the middle cerebral artery occlusion (MCAO) and reperfusion in rats on the evaluation of neurobehavioral function and on the volume ratios of brain infarction .Methods: Sprague-dawley (SD) rats were marked by group A or B; there were 10 rats in every group. The rats of group A subjected to temporary MCAO with heated round tip suture-embolus; the rats of group B subjected to temporary MCAO with glue-coated shuttled tip suture-embolus. After occlusion for 3 hours, the sutures were removed and the brains of rats were reperfusioned. The rats were evaluated the neurobehavioral function by a person who had known nothing about the group of the rats at 10minutes and 24 hours after reperfusioned. Then the rats were killed. The brains of the rats were removed and stained with 2, 3, 5-triphenyltetrazolium chloride (TTC), Calculated the infarct volume ratios through the results of TTC by the system of the image analyses. The results were compared between two groups.Results: The infarct volumes ratios were significantly larger in rats of group B than that of the group A (P<0.01). The infarct volume ratios of the rats of group A was tended to be smaller and more inconsistent than group B (coefficient of variation (CV) of the group A is 1.08, CV of the group B is 0.12). There is a liner correlation between the neurobehavioral function and the volume of brain infarction(r=0.873, P<0.01).Conclusions: To use the glue-coated shuttled tip suture-embolus to make the model of...