Preparation Of Polylactide Biomaterials Modified By Cyclodextrin And Evaluation For Its Cell Compatibility In Vitro

In this paper, the inclusion complexes (ICs) between polylactide (PLA) and cyclodextrin (CD) including a-cyclodextrin (a-CD) and β-cyclodextrin (β-CD) were prepared by the coordination effect under the ultrasonic and solvent condition. In addition, the copolymers of β-CD and lactide were also synthesized after the advanced protection of the hydroxyl in β-CD molecules through the trimethylsylation.Many analytical methods such as chemical analysis, Fourier Transform Infared Spectrum (FTIR), Nuclear magnetic resonance spectrum of ~1H (~1H NMR), Wide Angle X-Ray Diffraction (WAXD) and Thermal Analysis (TA) were employed for the characterization of ICs and the poly(lactide-co-β-cyclodextrin).The properties of the modified materials, including the thermal stability, hydrophilicity, degradation and cell compatibility were also studied. Results demonstrated that a-CD/PLA ICs exhibited amorphous structure and had lower thermal stability than a-CD, while β-CD/PLA ICs were partly crystal and more thermal-unstable than β-CD, and the two also possessed higher hydrophilicity compared with pure PLA.Poly(lactide-co-β-cyclodextrin) can be used to fabricate the porous scaffold with cavity diameter between 100-250 Mm. By comparing the weight loss percentage of the modified PLA and the PLA in phosphate buffer solution (PBS) (pH=7.4), we found that the modified PLA was with higher degradation rate than PLA. The mice fibroblast culture trial indicated that the modified PLA was an appropriate choice for the cell scaffold materials with good cell compatibility when the contact angle value of modified materials neared to 70°. All these results proved the PLA after modified with CD a promising scaffold material in tissue engineering.