Immune Condition And Correlation Of Immune Effect With DC-SIGN Gene Polymorphism In Infants Vaccinated With BCG

Objective To analyze the change in immune function and, to investigate the relationship between the immune effect and the gene polymorphism of DC-SIGN repeat region in Han infants vaccinated with BCG.Methods Peripheral blood specimen from 150 Han infants with positive PPD test (Group successfully Vaccination, SV) and 50 ones with negative PPD test (Group failure vaccination, FV) were collected. The cytokine IFN-γ and IL-10 concentrations were measured by ELISA to evaluate cell-mediated immunity function after BCG vaccination. The gene polymorphisms of DC-SIGN repeat region were determined by PCR and agarose gel electrophoresis to analyze correlation of BCG immune effect with the gene polymorphism.Results ①Serum IFN-γ concentrations in Groups SV and FV were 49.78± 12.20pg/ml and 38.08±7.68pg/ml, respectively (P=0.001). Serum IL-10 contents in Group SV and FV were 3.60±2.00pg/ml and 5.71 ±2.71 pg/m, respectively (P=0.008). ②There were two DC-SIGN alleles, i.e. 6 and 7, consisting of two idiotypes, i.e. homozygous 7/7 in 197 Han infants (98.5%) and heterozygous 6/7 in 3 Han infants (1.5%).③AII 150 individuals (100%) in Group SV had homozygous 7/7; Of 50 individuals in Group FV, 47 had homozygous 7/7 (94.0%) , but 3 had heterozygous 6/7 (6.0%) . Compared with individuals vaccinated successfully, a higher prevalence of variations of DC-SIGN repeat region gene was observed among individuals vaccinated failure (Fishers Exact Test, P=0.015).