Serial Studies On Preventive And Therapeutic Effects Of Hyperoxia Solution Against Hypoxemia

Objective1. To investigate the effects of intravenous hyperoxia solution (HO) on oleic acid induced ARDS rats.2. To study preventive effects of intravenous hyperoxia solution on hypoxemia after ordinary thoracic operation.Methods1. HO was prepared from Ringers solution. Thirty rats were randomly divided into three groups: Oleic acid group (group A), HO treatment group (group B) and control group (group C) with 10 in each group. ARDS model was established by iv oleic acid (0.1 mL / kg) in group A and group B, while in group C, the same volume of normal saline was given. At 30 min after oleic acid administration, HO(10ml/kg · h) was applied in group B, which was replaced by Ringers solution in group A and group C. Blood samples were taken for blood-gas analysis before and at 30,60,120min after oleic acid or normal saline administration. Two hours later, the animals were killed, the malondialdehyde (MDA) the superoxide dismutase (SOD) content in lung, content of lung water, lung/body ratio were measured, and pathologic changes of lung tissue were observed microscopically.2. Forty patients receiving ordinary thoracic operation were randomly divided into 2 groups: control group received normal balance salt solution and therapeutic group received hyperoxia balance salt solution. Solution in both groups was transfused 10 min after tracheal tube extraction. The blood gases in all patients were analyzed at 0 min, 120 min in transfusion and 120 min after transfusion. The vital signs were recorded and side reactions were observed at the same time.