Studies On QingDai And Bioactive Chemical Constitutes Of Compound Chinese Herbs Of CM

This thesis includes two parts. In the first part, the bioactive fractions of Compound Chinese herbs of CM were studied by means of activity track. Then the chemical constituents from the bioactive fractions were isolated. In the second part, one Chinese traditional medicine named Qingdai (Indigo naturalis) were investigated. The Optimum extraction method and solvent of extracting Indirubin from Qingdai and the chemical constituents of this medicine were studied. The main contents of this thesis are as follow:The water extract of Compound Chinese herbs of CM was provided by Chinese medicine institute of Guilin SanJin Pharmaceutical Co.,Ltd. During the course of investigation, the water extract was fractioned with different polar solvents, macroporous resin and silica gel chromatography. Both n-butanol parts and 10% ethanol parts of D-101 macroporous resin were proved to have pharmacological activities against platelet aggregation through experiment on rat's blood plasma in vitro. The results showed that n-butanol parts had exhibited obvious effect on inhibiting platelet aggregation which maximum aggregative rate was 3.06% at the concentration of 0.1249g/mL, while the maximum aggregative rate of 10% ethanol parts of D-101 macroporous resin were 6.76% at the concentration of 0.1660g/mL.To search bioactive constituents from Compound Chinese herbs of CM, we systematically investigated the chemical components of 10%~30% ethanol parts of D-101 macroporous resin. As a result, 3 compounds of triterpenoid Saponins were isolated by various means of separation, The acid hydrolysis of them yielded the same hederagenin and arabinose, rhamnose, glucose. The alkaline hydrolysis of A and C yielded the same glucose together with A'from A and C'from C, indicating their ester glycosidic linkage were two glcose. The structures of 3 triterpenoid Saponins were further identified on the basis of IR, FAB-MS, 1H-NMR, 13C-NMR and 2D-NMR (COSY, HSQC, HMBC, TOCSY) spectra, to be 3- O-α- L- rhamnopyranosyl (1→2) -α- L- arabinopyranosyl- hederagenin- 28- O-β- D- glucopyranosyl (1→6)-β- D- glucopyranosyl ester (A), 3- O-β- D- glucopyranosyl (1→3)-α- L- rhamnopyranosyl (1→2) -α- L- arabinopyranosyl- hederagenin- 28- O-β- D- glucopyranosyl (1→6)-β- D- glucopyranosyl...