Study On The Polymorphisms In The Gene For LDL Receptor-Related Protein And ATP-Binding Cassette Transporter-1 In Patients With Premature Coronary Heart Disease

Objective: To examine the distribution of gene polymorphisms for low density lipoprotein receptor-related protein(LRP) exon 22 C200T, ATP- binding cassette transporter-1(ABCA1) R219K and the association of these polymorphisms with lipid metabolism in patients with premature coronary heart disease(CHD).Methods: The premature coronary heart disease (CHD) group was comprised of 69 patients aged< 55 years in males or < 65 years in females with CHD proved by coronary angiography. The control group was 73 age matched subjects without CHD also proved by coronary angiography. All the patients' clinical data were collected. Genotypes and frequencies of LRP exon 22 C200T, ABCA1 R219K were examined by PCR-restriction fragment length polymorphism technique. All data were analyzed by SPSS 10.0 for windows.Results: The distribution of LRP exon 22C200T genotype differed significant between the CHD (CC: 62.3%, CT: 31.9%, TT: 5.8%) and control group (CC: 41.1%, CT: 52.1%, TT: 6.8%). The frequency of C allele was significantly higher in CHD than that in controls (P=0.036).The CHD patients with CC or CT genotype exhibited a significant increase in plasma TC and LDL-C concentration (P=0.007). C allele may be a risk factor for premature CHD (OR=1.909, 95% CI: 1.131~3.224, P=0.015). The distribution of ABCA1 R219K genotype and frequency of G allele did not differ between the CHD and control group. There was no association of the different genotype with plasma lipid level.Conclusion: The patients with premature CHD had higher frequencies of CC genotype and C allele in the exon 22 C200T polymorphism of LRP gene. The CC and CT genotype were associated with higher plasma TC and LDL-C level in CHD patients, while the gene polymorphisms for ABCA1 R219K were similar in the two groups and lack of association between the different genotype with plasma lipid level. The C allele of LRP exon 22 C200T may be a risk factor for premature CHD and the gene polymorphisms for ABCA1 R219K may have no relation with the premature CHD inChinese.