| Background: Pathological myopia (PM) is also termed malignant myopia or high myopia. It is usually defined as a refraction error equal to or above -6 diopters (D) in each eye,its a subtype of myopia,usually followed by the degeneration changes on the posterior pole of eye including scleral thinning ,choroidal atrophy and an abnormal lengthening of ocular axis.In all of the refraction error pathological myopia is most harmful, it is often accompanied with amblyopia,glaucoma,cataract,vitreous opacity and retinal detachmentthat can lead to dysphotia and blindness.re,recent findings indicated that the remodeling of scleral extracellular matrix(ECM) plays an important role in the development of pathological myopia, scleral collagen synthesis decreaseing and degradation increaseing lead to scleral thinning ,ocular axis length and overdistension of the eyeball.Sclera is the final target tissue of the eyeball control mechanism when myopia development and collagen accounts for most of scleral dry weight, the majority of this being collagen type I which has an important role in keeping the ground form of eyeball and preventing the development of pathological myopia . Therefore ,collagen type I is the object of this experiment,we studied the express change of collagen type I in pathological myopia scleral tissue extracellular matrix and express difference between the anterior and posterior pole scleral tissu extracellular matrix of normal human eyeball.Objective: This purpose is to analyze whether there is difference expression of collagen type I between pathological myopic eyes and non-myopic eyes scleral tissueextracellular matrix,and the anterior and posterior pole scleral tissue extracellular matrix of normal human eyeball,then to investigate the correlation with pathological myopia,and to provide new method for studying the pathological mechanism of maligant myopia and to provide the objective data for clinic.Methods: 1.1) To collect 15samples from the primary open-angle glaucoma POAG patients(with pathological myopia)who accepted trabeculectomy; 15 samples from the POAG patients without pathological myopia, who accepted trabeculectomy; 15 samples from the healthy volunteers anterior pole withoutpathological myopia, who donated their corneas; The sample was laminal slceral tissue during trabeculectomy. Among them the samples which obtained from the patients accompanied with pathological myopia were in the experimental group (EG),the others were in the control group (corneal transplantation CG and glaucoma CG)。2)To collect 15 samples from the healthy volunteers posterior pole, who donated their corneas, (corneal transplantation), 15 samples from the healthy volunteers anterior pole without pathological myopia(corneal transplantation).2.Extract the total protein form each sample and the expression of collagen type I in the experimental group and the control group were detected by using western blot , using UVP system to take picture and note the IOD ofβ-actin and collagen type I. In accordance with the electrophoresis strips'gray scale values ofβ-actin implored the ratios of the electrophoresis strips'gray scale values of collagen type I andβ-actin (collagen type I /β-actin).3.Using statistics methods such as analysis of variance ANOVA, Paired-Sample T Test and SPSS 11.5 software to assay the data.Results: The protein band of collagen type I was an 100 KD , there was significant difference between two groups(EG and CG)(P<0.001) and there was no significant difference between corneal transplantation CG and glaucoma CG (P>0.01).There is significant difference between corneal transplantation (anterior pole) and corneal transplantation (posterior pole) .Conclusion: Compared with CG ,the expression of collagen type I in EG decreased significantly and We got the condusion that the collagen type I was relative to the development of pathological myopia .But the mechanism of changes in expression of collagen type I was still to be studied further.
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