Autopsy Specimens Study Of The Benign Hyperplastic Nodules In The Peripheral Zone Of The Prostate

Objects: To investigate whether the benign hyperplastic nodules occurred in the peripheral zone of the prostate have something to do with that from the transition zone, we measured the distance between the nodules of the peripheral zone and that of the transition zone, and observed if there is any anatomic relationship between them. Moreover, we still studied the proportions of histologic components of the two kinds of nodules. Material and Methods: 1, Benign hyperplastic nodules specimens of TZ and PZ were obtained fromautopsy.2, From August 2004 to June 2006, we selected the prostate of the patients died not from the disease related to the prostate. After fixed with formalin for one week, we cut it perpendicular to the urethra, and got 12 hyperplastic nodules below the seminal colliculus in the outer gland tissue.3, We selected 12 hyperplastic nodules in the transition zone from the samepatient as the control group, and their diameter were similar to the PZ nodules..4, The distance between the nodules of PZ and the TZ tissue was measured.We also observed if there is any anatomic relationship between the nodules and the transition zone tissue with naked eyes.5, The pathologists in double blind diagnosed 12 specimens from theperipheral zone and the control group. 6, A combination of HE staining, Immunohistochemistry staining andcomputer-aided morphometric analysis were adopted to determine the percentage of stroma in the nodules and the percentage of smooth muscle, fibronectin, laminin, collagen types I, II, III, and IV in stromal tissue.Results:1, The nodules from the peripheral zone were white, round or oval shape, andwith clear boundary. The diameter of the nodules is about 4～9 mm (X|-±S,5.8±1.3mm). The distance between the nodules of PZ and the outer surface of TZ was about 2.5 to 5 mm (mean ± SD, 3.9±0.8 mm). There were no signs of anatomic connection between the nodules and the transition zone tissue.2, The percentage of stroma and epithelia in the nodules from transition zone was about 74.58±8.95%, 15.82±6.41%, and that of the nodules from peripheral zone was about 69.32±8.35%, 16.08±5.36%. There was no statistical significance between the two groups (P value was 0.15 and 0.91 respectively).3, The percentage of smooth muscle, fibronectin, laminin, collagen types I, II, III and IV in the stroma tissue have no statistical significances in the two groups (P>0.05)Conclusions:1, The benign hyperplastic nodules occurred in the peripheral zone maybeoriginate from the peripheral zone of the prostate and have nothing to do with the transition zone tissue.2, The percentage of stroma in the hyperplastic nodules from peripheral zonewas more than that of transition zone nodules, but there were no statistical significance between them.3, There was no statistical significance in histomorphology of stromabetween the two kinds of nodules.