Endothelial Ultrastructure Changes In Male Mouse With Castrated Or 5a -reductase Inhibitor

There exists a striking gender difference in atherosclerotic vascular disease. Two of the strongest independent risk factors for coronary heart disease (CHD) are increasing age and male sex. Despite a wide variance in CHD mortality between countries, men are consistently twice as likely to die from CHD as their female counterparts. Sex steroids hormone have been associated with cardiovascular diseases and the modification of the risk of CHD. Recently, there is controversy whether androgen has positive or negative function to cardiovascular disease. Once it was recognized that androgen in the male is predispose to atherosclerosis.Another was said that androgen hormone plays a very important role in vascular function. Androgen hormone has a protective effect in the arterial vascular system.Testosterone and dihydrotestosterone belong to androgen hormones. Dihydrotestosterone is produced from testosterone by 5 a -reductase. This study is to investigate whether low androgen hormone in which the rats were castrated and raised with 5 a -reductase inhibitor can caused vascular injury or not.Materials and Methods1 .Experimental animals and Methods: This study was conducted on 48 male Sprague Dawley rats. The rats were 5 weeks old, body weight 125±23g. They were divided into Group A: 12 rats were normal as control, Group B: 12 rats were castrated resulting in no or severe insufficient androgen, Group C: 12 rats were supplemented with testosterone undecanoate 50mg/kg/month, intramuscular injection after castrated. Group D: 12 rats were raised 5 a -reductase inhibitor to inhibit DHT. Rats were maintained in alternating cycles of darkness (6.00 pm to 6.00am.) and light (6.00am to 6.00pm). Food and water were freely available. 10 weeks after treatments described above. The rats were killed under the condition of intraperitoneal injection of ketamine (35mg/kg). During the operation, blood samples were taken for the measurements of T, FT and DHT by radioimmunoassay.2.Prepare and Observation of Aortal Endothelial Cell under Scan Electron Microscope including (1) Washing tissue. (2) Chemical fixation. (3) Chemical dehydration.(4) Drying.(5) Exposure artery endothelium: open under light microscope and emerge the endothelia with microsurgery scissors. Handling was gentle, delicate and agile. (6) Conductivity enhancement (7) All samples were observed and photographed under the equipment of scanning electron microscopy.3.Statistical analysis: experimental values were expressed as means± SD, Analysis of variance was done by SPSS10.0 for window One-Way ANOVA.ResultsThe serum T and Free T concentration in group B were significantly lower than that of group A, C and D (P<0.01); The DHT in group D were decreased remarkably (P<0.05) comparing in group A and C. The endothelial ultrastructure in groups B and D with insufficient male hormone were severely damaged such as crimple, coarse, protuberant and the broken in connection area.ConclusionsInsufficient of testosterone and dihydrotestosterone led to endothelial ultrastructure destruction in rats.