The Influence Of The IHCP Combined Vein Chemotherapy For The Cancer Of Stomach, Immune Function And HSP70

OBJECTIVESObserving the different effects between the IHCP combined with vein chemotherapy and the conventional vein chemotherapy; Comparing with two methods of treatment to stomach cancer patient immunity function influence difference and the HSP70 value change; And analyzing wether or not there is relevance between the value of HSP70 and the T cell subgroup. We may seek the more reasonable treatment plan by all these, and explain possible mechanism --thermotherapy to patient immunity function influence in molecular level.METHODSThe two groups of patients use the standard chemotherapy plan. The group of IHCP combined with vein chemotherapy were carried on intraperitoneal hyperthermia chemop-erfusion with PDD. We must heat the patients with RF immediately after the IHCP. The platform temperature must not be lower than 41℃,and the time must be more than 60 minutes. The other medicines may carry on vein chemotherapy according to the conventional usage. We can do statistical analysising to the curative effect after the two cycles, and then compare with them if there is difference. We may withdraw the serum samples before and after the treatment and examine the T cell subgroup and HSP70' value. Through comparing the value with the normal people's and to show if there is changing in the aspect of the patients, immune function and the HSP70 value. Finally, we carry on one-factor analysis of variance to each group of date and relative analysis to the HSP70 value and T cell subgroup.RESULTS1 We can make analysis to the curative effect and carry on chi-square test after two cycles. The result shows that the IHCP combined with vein chemotherapy is better than conventional vein chemotherapy. And the difference between them has statistic significance(P<0.05).2 Comparing the two groups with normal group around the treatment, We can get the results: The discrepancy of CD3+T cell,CD8+T cell and CD4 + T/CD8+T cell has remarkable statistical significance(P<0.01) by comparing the control group before and after therapy and the curative group before therapy with the normal group; And the discrepancy of the CD4+T cell has statistical significance(P<0.05) by comparing the two groups before therapy with the normal group through one-factor analysis of variance. But for the curative group before therapy and the normal group, the discrepancy has no statistical significance. Then we analyse the two groups and do t test around treatment. The curative group's values have remarkable statistical significance(P<0.01) by comparing the lever of T cell subgroup before therapy with the lever of it after therapy, but the control group's values have no statistical significance (P > 0.05)by comparing before therapy with after therapy.3 We test the two groups' HSP70 value around the treatment, then compare the results with the normal group. The HSP70 value of the curative group after treatment is higher than the normal group. And there is statistical significance (P<0.01). But there is no statistical significance(P> 0.05) for the other groups. The HSP70 value of the curative group after treatment is higher than before treatment and there is statistical significance (P<0.01). But there is no statistical significance(P> 0.05 )for the control group after and before treatment.4 Taking HSP70 as the independent variable, and respectively take value of the CD3+T, CD4+T, CD8+T and CD4+/CD8+T as the dependent variable, we can get the r value respectively is 0.602,0.8,-0.751 and 0.76(the P fig. are all less than 0.01).And there is correlation between the HSP70 value and CD3+T,CD4+T,CD8+T and CD4+/CD8+T: The HSP70 value and CD3+T,CD4+T and CD4+/CD8+T presents positive correlation; but the HSP70 value and CD8+T shows negative correlation.CONCLUSIONS1 The abdominal cavity and the pelvic cavity tumor hold the very great proportion in all malignant tumors. The main reason of the failure treatment is dissemination and metastasis. The probability of the tumor dissemination is higher especially for the patients that the tumor had penetrated into the placenta percreta. The IHCP can improve the concentration of the medicine in belly cavity. And the medicine may display desired effect when it is heated. More important thing is that the curative effect is better than conventional vein chemotherapy. Comparing the two methods, the effect of the IHCP combined with vein chemotherapy is better. The discrepancy has statistical significance (P<0.05). This gives us a good treatment plan for the tumor growed in the belly and pelvic cavity.2 The patients suffered tumor often create the immunity function lowly because of own immune deficiency or the tumor suppression immunity function. We can find all the patients+ immunity function low through testing the T cell subgroup of the two groups: The CD3+T,CD4+T,CD4+T/CD8+T is lower than the normal control group,s; But the CD8+T is higher than the normal control group,s (P<0.05). Thermotherapy may improve the patients, immunity function and enable it to approach the normal control group after the IHCP combined with vein chemotherapy. The value of the T cell subgroup compared with the normal control group,s has no statistical significance. And after treatment, the immunity function can get enhanced. It presents that the CD3+T, CD4+T and CD4+T/CD8+T is higher than that of before treatment, while the CD8+T is lower. But for the conventional vein chemotherapy, the immunity function after treatment is lower than before. And it has no statistical significance. Thus it can be seen, the heating may enhance patients, immunity function. And this may be the further theory basis to enhance the curative effect based on hot sensitization.3 HSP70 is a kind of irritability protein that is produced when the cell is being heated and damaged .It plays a complex and vital role in the process of the thermotherapy. The HSP70 mainly produces in the cell, but there is certain content in the serum. Around two groups of patients, treatment, the content of the HSP70 in the serum have both improved. But for the group without heating, the improvement of the HSP70 value has no statistical significance(P > 0.05 ). While the HSP70 in the serum may obviously improve after treatment, comparing with before treatment and the discrepancy has statistical significance (P<0.05). Thus we can see that chemotherapy and heating can both enhance the HSP70,s expression, and the main reason is heating. 4 How about mechanism of action the thermochemotherapy improve the immune function is one of the contents we study now. We can find that there is correlation between CD3+T,CD4+T,CD8+T and CD4+/CD8+T and the HSP70, expression by correlation analysis: The HSP70 and the CD3+T, CD4+T and CD4+/CD8+T presents positive correlation; But the HSP70 and CD8+T shows negative correlation. All these show the HSP70 may improve the immune function. In other words, the molecule basis of the thermochemotherapy can improve patient,s immune function is possible due to producing of the hot shock protein and acting on the immune system then.