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Study On The Expression And Molecular Regulation Mechanism Of CD44 In HL-60 Cell Induced By Homoharringtonine

Posted on:2008-08-03Degree:MasterType:Thesis
Country:ChinaCandidate:C P YanFull Text:PDF
GTID:2144360212494249Subject:Internal Medicine
Abstract/Summary:PDF Full Text Request
ObjectiveTo explore the change and mechanism of CD44 and the relation between CD44 and cell cycle regulation in HL-60 cells induced by homoharringtonine. MethodsHL-60 cells were incubated with HHT for 1 to 3 days; cell morphous were observed by Wright's staining; cell proliferation were detected by MTT; cell differentiation were detected by NBT; cell differential antigen CD11b and cell cycle were detected by flow cytometry; and CD44,p27,p21,Cyclin E gene mRNA were detected by RT-PCR. ResultsThe proliferation were inhibited and the differentiation were promoted in HL-60 cells induced by HHT. RT-PCR reaction showed that CD44 and CyclinE mRNA decreased and p27 and p21 mRNA increased; furthermore, there was a negative correlation between the expression of CD44 and p27 mRNA. ConclusionsHHT played a role in the differentiation of HL-60 cells that may be mediated by the down-regulation of CD44, and then up-regulated p27, p21 and inhibit the activity of cyclin E.
Keywords/Search Tags:HHT, CD44, cell line, HL-60, induce differentiation, cell cycle regulation
PDF Full Text Request
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