Treatment Of Spinal Cord Injury By Transplanting Cholinergic Neurons After Inducing And Directionally Differentiating Neural Stem Cells In Vitro

Objective: To observe the effects of transplanting cholinergic neurons after inducing neural stem cells in vitro and differentiating them into cholinergic neurons on treating spinal cord injury.Methods: Adopt hippocampus isolation of transgenic fetal rat (12-14d) to culture neural stem cells and induce them to differentiate into cholinergic neurons. SCI models were made by transecting Spinal cords of Adult Sprague- Daweley rat with microscissors. 18 Sprague - Daweley rats were divided randomly into three groups. Group A is the control group which was injected DMEM/F12. Group B is the experimental control group which was injected cell sap of neural stem cell. Group C is the treatment group which was injected cell sap of cholinergic neurons. Locomotor functions of three groups in the experiment were detected termly by BBB score after cell transplantation. By the end of the eighth week after transplantation, the spinal cord tissues were extracted. Frozen sections were carried out by immunohistochemical stain of ChATResults: Cell line isolated from the hippocampus has ability of proliferation and expresses nestin. Extract from fetal rat skeletalmuscle can induce 11.8% of the isolated cell line to differentiate into cholinergic neurons. This is different from the control group (P<0.01) significantly. BBB scores of all the adult rats in group B and C were obviously higher than that in group A after 3 weeks after transplantation (P<0.05). BBB score in group C was obviously higher than that in group B after 4 weeks after transplantation (P<0.05). By the end of the eighth week after transplantation, ChAT positive cells were observed in the frozen section.Conclusions: NSCs can differentiate directionally into cholinergic neurons in vitro. Transplantation for treating spinal cord injury, can stimulate the recovery of motor functions of injuried spinal cord.