| Objects: To expore the useful value of bone marrow mesenchymal stem cells, neuron growth factor and adenosine triphasphate on rat cortical neurons after anoxia-reoxygenation in vitro.Methods: Bone marrow mesenchymal stem cells were isolated, cultured and expanded, the culture medium was substituted by new medium that contain no serum when cells grew to 90% confluence. After cultured for 24 hours, the medium was collected as BMMSCs conditioned-medium (B-CM), Cortical neurons of mouse cultured for 8 days were divided into five groups. 1) Normal control groups, 2) Hypoxic groups, 3) B-CM interfered groups, 4) NGF interfered group, 5) ATP interfered group. Neurons were cultured for 24 hours after anoxia for 6 hours except that of normal group, Cell viability were detected by MTT and LDH methods, the apoptosis of neurons was examined by flow cytometry and telemicroscopy after anoxia for 6 hours and anoxia-reoxygenation for 24 hours. Results: Compare with normal control group, the hypoxic cell viability increased and the leakage of LDH and the ratio of apoptosis were decreased(P < 0.001); bone marrow mesenchymal stem cells and adenosine triphasphate could improve the cell viability(P < 0.001), decrease the leakage of LDH(P < 0.01) and the ratio of apoptosis (P < 0.01) compared with anoxia group on 6 hour; bone marrow mesenchymal stem cells, adenosine triphasphate and neuron growth factor could improve the cell viability(P < 0.01),decrease the leakage of LDH(P < 0.05) and the ratio of apoptosis (P < 0.05) compared with anoxia group on 24 hour. Bone marrow mesenchymal stem cells and adenosine triphasphate have more remarkable effects compared with NGF treatment(P < 0.05).Conclusions: Anoxia could damage cortical neurons and induce apoptosis, bone marrow mesenchymal stem cells, neuron growth factor and adenosine triphasphate could improve the cell viability and inhibit apoptosis of anoxiated neurons.
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