Research Of Preparation And Biocompatibility Evaluation Of Nanosized As₂O₃/Fe₃O₄ Complex And Its Theraputic Effect Of Thermochemotherapy On Cervical Cancer

[Objective]â‘ To study the preparation and characterization of Fe₃O₄ magnetic nanoparticles and nanosized As₂O₃/Fe₃O₄ complex.â‘¡Thermodynamic test of various doses of Fe₃O₄ magnetic nanoparticles and nanosized As₂O₃/Fe₃O₄ complex.â‘¢To evaluate the biocompatibility of Fe₃O₄ magnetic nanoparticles and nanosized As₂O₃/Fe₃O₄ complex.â‘£To investigate the therapeutic effects of thermochemotherapy of nanosized As₂O₃/Fe₃O₄ complex on the cultured cervical cancer Hela cells.⑤To investigate the therapeutic effects on xenograft cervical cancer treated by thermochemotherapy of nanosized As₂O₃/Fe₃O₄ complex.â‘¥To study the inhibition of the gene expression related cancerometastasis treated by thermochemotherapy of nanosized As₂O₃/Fe₃O₄ complex.[Methods]â‘ Fe₃O₄ magnetic nanoparticles were prepared by the technique of chemical co-precipitation and nanosized As₂O₃/Fe₃O₄ complex was prepared by the impregnation process, their characteristics were observed by means of transmission electron microscope(TEM), x-ray diffraction(XRD), scanning electron microscopy(SEM), energy dispersive spectrometer(EDS) and computer color magic image analysis system.â‘¡Thermodynamic test was used to observe temperature change of various doses of Fe₃O₄ nanoparticles and nanosized As₂O₃/Fe₃O₄ complex under alternating magnetic field (AMF).â‘¢MTT assay, haemolysis test, micronucleus test, and LD50 calculation of mice were used to evaluate the biocompatibility of Fe₃O₄ nanoparticles and nanosized As₂O₃/Fe₃O₄ complex.â‘£The therapeutic effects on cultured Hela cells treated by thermochemotherapy of nanosized As₂O₃/Fe₃O₄ complex were evaluated by inverted microscope, electron microscope, MTT test and flow cytometry.⑤After injected into xenograft human cervical cancer of nude mice respectively, nanosized As₂O₃/Fe₃O₄ complex was exposed to alternating magnetic field for three times. 6 weeks later, the inhibition rate of the mass and volume of the tumors was calculated. At the same time, histological changes of tumors of tumor-bearing nude mice were observed under light microscope.â‘¥To study the potential mechanism of anti-cancerometastasis of thermochemotherapy of nanosized As₂O₃/Fe₃O₄ complex, the expression of CD44v6, VEGF-C ,and MMP-9 were detected by RT-PCR technology .[Results]â‘ The self-prepared Fe₃O₄ nanoparticles are approximately spherical, high electron-dense, uniform in size and have good dispersibility observed TEM, which average diameter is about 20nm...