| Chronic Myelogenous Leukemia is a common malignant proliferous disease in hematology, next to AGL and ALL. The most obvious genetic marker in WBC of CML is a specific Philadelphia chromosome (Ph). This Ph is formed by translocating chromosome No.9 to No.22, forming two fused genes, namely ABL/BCR and BCR/ABL. A kind of microprotein with molecular weight 210 000 (P210bcr/abl ) may be expressed by the latter gene. More than 95% CML patients have this protein in WBC. Detecting this protein is an important way to diagnose CML accurately and specifically. Based on this theory we develop this studies.The new method is established, which is suitable to the conditions of our laboratory for detecting the P210bcr/abl from WBC in the peripheral blood of patients with CML and for diagnosing CML specifically, sensitively and rapidly. Blood sample from the peripheral veins was collected. The protein (P210bcr/abl ) in WBC of CML was isolated with SDS-polyacylamide gel electrophoresis. The separated substance is transferred to the NC membrane by Western blotting assay and combined with a special monocloned antibody, forming Ag-Ab complex. The protein zones on NC membrane were treated with ECL, and then exposed to the X film. The results are qualitatively reviewed, according to blots appeared on the film. We used this method to detect 68 cases of CML patients who were conformed to FAB typing and 20 normal persons. The results of the patients were all positive. There was agreement with M.D.Anderson cancer center's results. The results of the test are very accurate, sensitive and specific for diagnosis of CML, even as few as 0.2% Ph positive cells can also be detected. The test is timesaving. It turns around of only 2 days. The results can be faxed to physicians immediately.The test is very convenient, with only 10cc haparinized peripheral blood required and no pain for patient. Therefore, this method is worth using in clinical condition.
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