The Protection Of Retinervus Luffae Fructus And The Saponin Extraction From Retinervus Luffae Fructus In Myocardial Ischemic Injury

OBJECTIVES:To observe the effect of Retinervus luffae fructus (RLF) on acute mycocardial ischemia in mice. We examined cardial function, myocardial enzymology and changes of myocardial struction, and tried to find the feasibility of RLF against myocardial ischemia and its mechanism, and provided foundation of new thoughts and experiments for acute myocardial ischemia. We erected the saponin extraction from RLF, and used the model of hypoxia to investigate the function and mechanism of the saponin extraction from RLF. METHODS:1. Pituitrin was used to establish the model of acute myocardial ischemia in mice, and was investigated the effect of RLF on cardial function. By comparing with Dansan pill, the changes of the T wave, heart rate and interval of P-R of electrocardigram in myocardial ischemia mice were observed.2. After 30 min myocardial ischemia, serum and myocardium of the mice were restored. Not only the content of lactose dehydrogenase (LDH) in the serum but also the activities of superoxide dismutase (SOD) and maleic dialdehyde(MDA) in the myocardium were examined. And the content of NO in the serum and in the myocardium was also examined. Meanwhile, myocardial paraffin wax slices were made and HE staining was used to observe the changes of tissue micro-structure.3. The saponin extraction from RLF was separated from the water of RLF mixture with D101 silica gel column. The optimum ethanol concentration was 60%.4. Neonatal of SD rat hearts were used to establish primary cultured cardiomyocytes. The toxicity of the saponin extraction from RLF was judged by MTT, and the IC50 was ensured. By using the cardiomyocytes subjected to hypoxia, we detected death rate and total antioxidative capacity of cardiomyocytes subjected to hypoxia.