Synthesis And In Vitro Transfection Study Of Linked PEI With Low Molecular Weight

This thesis study address the issue of transfection efficiency and toxicity of polycationic gene carrier by a rationally designed biodegradable polyethylenimine ( PEI ) derivative. This new PEI derivative ( named PolyLinkB, BLP) was synthesized by conjugating low molecular weight PEI ( Mw = 800 Da ) with 1,4-Butanediol bis-(chloroformate) sa the linker. BLP showed significantly high transfection efficiency than PEI25K (...