Study On The Binding Of Small Molecules To DNA And DNA Bases As Well As The Effect Of CdS Nanoparticles On The Binding Behavior

Many genetic mechanisms in human bodies are all about recognition of DNA. It attracts continuing interest in research areas of biology, chemistry, pharmacy since some small molecules may recognize specific sequence of DNA which may be related to some dangerous illness like cancer and so on. With the development of human genome; we will know more about life phenomenon which will promote research on the development and promote research on gene-targeted drugs. New generation of gene-targeted drugs are recognition, incision and repairing of active biological molecular based on molecular level. The new treatment is an effective treatment of the disease caused by genetic mutation.In this study, we have initially explored the binding of procaine hydrochloride to single-stranded DNA (ssDNA), double-stranded (dsDNA), and DNA bases by using pulse voltammetry. The results indicate that the procaine hydrochloride binds to dsDNA with a groove binding mode, and the interaction of procaine hydrochloride with dsDNA to is stronger than that with ssDNA. Besides, the binding of between procaine hydrochloride to purines is stronger than that with pyridines and the binding affinity with different DNA bases is in the order of G>A>T>C.In addition, we have investigated interaction of MB or MG with DNA and DNA bases by using UV–vis Spectroscopy. The results indicate that the interaction between MB or MG with different DNA bases is recognize strong, and MB can recognize single-stranded DNA from double-stranded DNA while MG can not recognize it.Finally, the binding of procaine hydrochloride, MB, MG to DNA and DNA bases in the presence of CdS nanoparticles has been explored in our work by using pulse voltammetry and UV–Vis absorption Spectroscopy. The results show that the presence of CdS nanoparticles can enhance the probing sensitivity for the interaction between procaine hydrochloride and DNA or DNA bases and that between MB or MG with DNA and DNA bases. This relative study may provide some fresh information for the development of new gene-targeted drugs.