The Correlation Between The Tumor Surrounding Tissue Invasion And Immunocyte Changes In Breast Cancer

Objective:To investigate the immunocyte changes in human breast cancer and in tumor surrounding normal tissues, how the cell changes in quality and quantity in different conditions, such as different grades, different size of tumors, positive or negative of metastases of axillary lymph nodes, and different ER, PR expression, different tumor invasive depth, and the relationship between these changes and the patients prognoses.Methods:1. Samples and HE dyeing: the tumors and tumor surrounding normal tissues were prepared form our clinical patients, the samples were preserved in minus 80 celsius degree in 30 min after they were resected in operations, then the samples were paraffined, slice up into 3μm. Again pathological diagnoses of each sample and its invasive depth were conformed after HE dyeing, but before the immunohistochemistry assay.2. Immunohistochemistry detection of immunocytes in the tumors and tumor surrounding normal tissues: the changes of quality and quantity of dendritic cells, T lymphocytes, B lymphocytes, macrophages were detected by immunohistochemistry, and the relationship between these changes and different grades, different size of tumors, positive or negative of metastases of axillary lymph nodes, and different ER, PR expression.Results:1. Tumor size and tumor invasive depth were related closely(p<0.05). Tumor invasive depth were also different in I, II, III grade of diseases (p<0.05).2. The relations between the quantity of invasived dendritic cells and the clinical data of breast cancers: positive dyeing dendritic cells distributed with T lymphocytes not only in the tumor surrounding, but also in the tumor, among the cancer cells, thecell tubers contacted with cancer cells directly. Statistical analysis suggested: the quantity of invasived dendritic cells reduced in breast cancer with more axillary lymph nodes metastases, more sever disease grades and more invasive depth(P<0.05). The quantity was also related to c-erbB-2(P<0.05), but did not related to ER or PR expression(P>0.05).3. T lymphocytes invasion: the quantity of invasive CD45+T lymphocytes was correlated to the metastases of axillary lymph nodes (P<0.05), but have no relation with the grades and tumor sizes.4. The relations between the quantity of invasive macrophages and the clinical data of breast cancers: the quantity was correlated to differentiate grades, depth and metastases of axillary lymph nodes. Invasive macrophage quantity was significantly higher in I, II grade breast cancer than III grade (P<0.05).5. B lymphocytes: CD20+B lymphocytes were distributed among the cancer cell, the quantity was correlated with invasive depth and axillary lymph nodes (P<0.05)Conclusion:1. The breast cancer invasion in nipple direction was more severe than in other directions. There was a negative correlation between the invasive depths and the other factors such as axillary lymph node metastases, grades and c-erbB-2 expressions. Before the breast conservation operation, the factors could be used as the predictors to evaluate the operation extension and the breast re-building technique.2. The clinical grades were negatively related to the quantity of dendritic cells in the tumor, suggested the dendritic cells reduced along with the development of breast cancer. The quantity of dendritic cell predicts the clinical characteristics and metastasis conditions, facilitates high-risk patients selection and effective therapy.3. T lymphocyte could be used as a predictor in predicting the breast cancer clinical characteristics and metastases, because its quantity was related to axillary lymph nodes metastases. As the major downstream cell of dendritic cell, T cell changes were as same as those of dendritic cell, it might reflect changes in quantity and function of dendritic cells.4. The quantity of macrophage invasion might predict the poor clinical outcomes of the breast cancer patients, because of the correlation of cancer metastases and invasion. TAM boosts the cancer occurrence, invasion and metastases, instead kills the cancer.5. B lymphocyte plays an anti-carcinoma role by inducing the immunologicalreaction, suppresses the proliferations and metastases of the breast cancer.