Culture And Identification Of Mesenchymal Stem Cells From Rabbits Bone Marrow In Vitro

Popurse: To establish a method of in vitro isolation,purification and amplification of bone marrow derived mesenchymal stem cells of adult rabbits,for a further study .Methods: Bone marrow tissue was harvested from tibias bone of rabbits,mesenchymal stem cells were isolated by the method of density gradient centrifuge.Then MSCs were proliferated by adhering culture . Growth curve of 2th,3th,4 th,5th,6th generation MSCs were drawn . CD34,CD45,CD105 antigen of MSCs were identified by Flow cytometry.Results: Primary cultured MSCs start adhering to plates 12 hours after seeding, and spindle-shaped MSCs were observed under microscopy, and growing at a rapid speed .Confluence of MSCs reach to 80% 7-10 days after seeding.From the MSCs growth curve ,which was S shape ,it was known that MSCs grew in a rapid stage at 3th-7th generation after seeding, and MSCs of 3th-5th generation possess higher activity . CD34(-),CD45(-),CD105(+) were directed.Conclusion: In this study, by our culture protocol ,MSCs were isolated and proliferated stably and rapidly .Cultured MSCs possessed high activity of growing and amplification,and were appropriate for further research.