| Objective and Methods: The exact relationship between OLP and herpesvirus, especiallyβ-herpesviridae family, is still unknown now. The aim of this study was just to survey that. It was proved by those previous studies about OLP disease with herpesvirus, which mainly employed classical PCR assay, that the positive rate of HHV-6 and HCMV was relatively higher than other herpesvirus in the biopsies from OLP sections. According to the higher positive rate of that and the characteristics of relapse of OLP, some researchers presumed that some herpesvirus, such as HHV-6 and HCMV, may be as a potential cause result to OLP, but that hypothesis still was inconclusive at statistics. In addition, though classical PCR to clinical tissue samples was used most frequently until recently, it is insufficient to estimate the condition about replication of active virus. Moreover, previous OLP studies associated with herpesvirus by classical PCR have some defections at the selection of clinical samples, so we have selected 16 patients which might be tightly contacted with herpesvirus infection and employed 10 healthy controls, and meanwhile we have enriched the breed of clinical samples being involved in WBC, serum, saliva, exfoliated cell, and biopsy samples, respectively. It is not only to make best use of the real-time PCR technology, but also to be in line with the statistics principle in our present study, accordingly. Finally, the real-time PCR assay, whose Cycleave fluorescence probes we employed made by hybridization of RNA & DNA and combined with RNase H when itwas put to practice, would be an ideal method in our present study. The previous experience of which has reported that it had comparable sensitivity, except for superior reproducibility and precision, compared to the others quantitative PCR assays.Results: Destination viruses were detected in 27 of 85 (31.6%) samples from 14 of 16 (87.5%) patients with OLP and in 5 of 40 (12.5%) samples from 3 of 10 (30%) healthy controls. Seven times of HHV-6 and twenty-seven times of HCMV were quantified from 85 samples of patients with OLP, and one time of HHV-6 and five times of HCMV were quantified from 40 samples of 10 healthy controls. HHV-6 were quantified eight times (OLP 7, Healthy controls 1), and HCMV thirty-two times (OLP 27, Healthy controls 5). Both viruses (HHV-6 and HCMV) were simultaneously detected in 4 patients with OLP (from 6 samples) and in 1 healthy control (only from 1 sample). The HCMV positive rates of each kind of samples is, respectively, WBC50%(8/16), saliva 25%(4/16),biopsies 80%(4/5), exfoliated cells from un-lesion sections 18.75%(3/16); the counterpart in healthy controls is, respectively, saliva 20%(2/10), WBC30% ( 3/10 ) and exfoliated cells 0%(0/10). The destination viruses were not detected in all serum samples.Conclusions:①Chi-square test (X2=6.8290,P<0.05)and the value of Pearson contingency coefficient (0.2080) showed that HCMV was significantly closely related with patients with OLP.②Exact probability test (P=0.0028) showed that the HCMV positive rate in un-lesion exfoliated cells of OLP group has significant statistics difference with their saliva samples, otherwise the HCMV positive rate in biopsies has not significant statistics difference with its WBC samples (exact.sig. P =0.40), so we presumed that the exfoliated cells from lesion sections might be a better way to study patients with OLP, especially in viruses.
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