The Research About The Relationship Between Myosin Light Chain Kinase Expression And Inflammatory Bowel Disease Activity

Background:The intestinal epithelial barrier is frequently disrupted in inflammatory bowel disease (IBD) and this has been proposed to play a role in disease pathogenesis and reactivation. In vitro studies show that cytokine-induced epithelial barrier dysfunction can be mediated by increased myosin light chain kinase (MLCK) expression.A primary function of intestinal epithelia is the maintenance of a barrier to prevent unregulated mixing of luminal and interstitial compartments. While the relevance of epithelial barrier dysfunction to disease pathogenesis remains a subject of active investigation, it is well accepted that compromised barrier function is a common feature of infectious, ischemic, and immune-mediated intestinal disease.Objective: To discuss myosin light chain expression in inflammatory bowel disease.Methods: Biopsy specimens were obtained from patients undergoing colonoscopy for IBD,10from ulcerative colitis and 10 from Crohn's disease. Control samples were obtained from patients undergoing colonoscopy as part of a routine program of polyp surveillance. The number of control samples is 10.Then to detect the myosin light chain expression,the above samples are in immunohistochemical staining.At last we calculate the rate of positive cells through the microscope.Results: The rate of positive cells in Crohn's disease is 50.0% ±10. 0%, the rate of positivecells in ulcerative colitis is 57.6%±25. 7%, the rate of positive cells in control samples is 9. 2%±2.7%. the difference between Crohn's disease and control samples is significant., (P<0. 01) sois the difference beween ulcerative colitis and ulcerative colitis. (P<0. 01)Conclusion: epithelial myosin light chain kinase expression is upregulated in inflammatory boweldisease.