Protective Effects Of Aprotinin On Acute Alcohol-induced Liver Injury

The studies on the mechanism of liver injury and its medicine treatment have become the hot spot in domestic& oversea biology and medical area. Aprotinin is a serine proteinase inhibitor which is extracted from bovine lungs.It is effective in preserving platelet function,anti-inflammatory, anti-fibrinolysis. Aprotinin has been successfully used in arresting bleeding during surgery, pancreatitis, shock, asthma and gynecological illnesses etc. The current findings suggest:aprotinin has protective effects in many experimental ischemia-reperfusion (I/R) injuries such as liver, brain and heart injuries.This paper will use intragastric administration of alcohol to build the acute hepatic injury and use intra-peritoneal injection of aprotinin to protect rats liver. And we will discuss the mechanism for its protection of liver injury.60 healthy adult Wistar rats, male and female each occupying one half, were randomly divided into four groups:the blank group, the model group and the high-dose aprotinin group,the medium-dose group,the low-dose group, the positive control group, with 10 rats in each group.The positive control group,the model group and the high, medium, low-dose aprotinin groups were intragastric administrated with alcohol in 8:00 am,while the blank group were administrated with the same volume of water.At 8th day after we built the model,we used intra-peritoneal injection of aprotinin, positive medicine in the homologous group in 2:00pm, respectively, while theblank group and the model group were injected of water. This lasted to the end of experiment. After the last administration, rats were banned of the diet but water for 12h. The blood samples from the abdominal aorta were obtained after anesthesia and deposited at room temperature for 1h, and then centrifuge 3000rpm for 10min. after that, we transferred the supernatant into tubes, then the plasma ALT,AST,SOD,LPO,GSH-Pxcontent;the liver SOD,LPO content; Liver weight coefficient;pathological change of rats liver were detected. All the records were indicated by mean value standard deviation( x±s),all data in the two groups were analyzed with T-test.Results showed that compared the model control group and normal control group, the serum ALT,AST and LPO increased significantly (p<0.001),the serum GSH-Px,the serum and liver SOD decreased significantly(p<0.001),the model control group liver weight coefficient increased significantly(p<0.001),the liver LPO increased significantly (p < 0.001).The color of model control group liver tissue became lighter and the surfaces of rats'livers were coarse, and the brittle particles were seen and showed congestion and fat denaturation, the inflammation cells have been infiltrated.Compared with the medium, high -dose aprotinin groups and model group, the serum ALT, AST and LPO decreased significantly (p<0.05),the serum GSH-Px,the serum and liver SOD increased significantly (p<0.05),the model control group liver weight coefficient decreased significantly (p<0.05),the liver LPO decreased significantly (p<0.05). Pathological changes of rats liver alleviated gradually compared with model group, fat denaturation became less but congestion still be seen. there were some necrosis of hepacytes and infiltration of inflammation cells.The results showed that: The rats'acute liver injury experimental model built successfully.The intra-peritoneal injection of aprotinin could protect the rats'liver from acute liver injury very effectively.In conclusion,the rats'acute liver injury experimental model built successfully with intragastric administration of alcohol,and then we observedthe protective effects of intra-peritoneal injection of aprotinin on acute alcohol-induced liver injury.The results prove that aprotinin have the explicit protective effects on acute liver injury and new efficient way of drug taken,show its application prospects of aprotinin on the treatment of multi-organ failure,and provide the powerful evidences for its application in the areas of medicine.