| ObjectiveTo investigate the relationship between the extent of blood–retina barrier ,the expression of VCAM-1,E-selectin in the retina and sVCAM-1,sE-selectin in the aqueous humor and vitina.the expression of VCAM-1 in the retina,the level of sVCAM-1 in the aqueous humor and vitina,the extent of blood-retinal barrier and the aneurysms,pericyte ghosts,acellular capillary sidanohphilia were detected intervened by Aminoguanidine. Methods:in our expriment,we divided all Wistar rats into six groups:control group(M),diabetes 3 months group(M1), diabetes 5 months group(M2), diabetes 7months group(M3), diabetes 9 months group(M4), diabetes 11 months group(M5),we made a diabetes animal model with one-dose injecting STZ through intraperitoneal injection method,the concentration of blood glucose was adjusted by inject insulin to guarantte the comparition among such groups(P>0.05).the concentration of blood glucose ,urine glucose, HbA1 and the concentration of Aminoguanidine in blood were monitored once week,those rats that the concentration of blood glucose exceed 16mmol/L and weight decrease obviously but chang not so much in each parallal group were recruited.We detect the expression of VCAM-1 and E-selectin of retina in each group to made an animal model which such protein mobiled with time . We chose Aminoguanidine to intervent the development of DR,dividing diabetic rats into control group(3 and 11 month) ,4 eyes in every group,treated by Aminoguanidine 25mg/kg,50mg/kg,75mg/kg and 100mg/kg ,4 eyes in each group,randomly.dividing treatment group into early treatment and advanced treatment group further,Aminoguanidine(50mg/kg,twice every day)was given after injecting STZ to maintain 3 months in early group, Aminoguanidine were treated 8 month after injecting STZ to maintain 3 months in advanced group.the level of sVCAM-1 in aqueous humor was used to monitor the intervention effect of Aminoguanidine to suppress the adhesion molecule of DR rats,compare the forecasting expression value of retina to the actual value ,evaluate the predicting ability of the regression equation. Results:1,we found that the expression of VCAM-1 and E-selectin in retina became stronger with time ,the expression extent shows obvious time correlation after mading model. all these protein increased in the 3th month after STZ injected,compared with control group,the difference show significance(P<0.05);2,sVCAM-1 is more sensitive than sE-selectin in aqueous humor and vitrina,it began to increase after 3th month injected by STZ,howerve, sE-selectin began to elevate after 7th month compared with sVCAM-1, the tendency show same with the expression of VCAM-1 in retina.3,the extent of the blood–retina barrier destroy became stronger with time and shows prominent correlation with the level of sVCAM-1 in the aqueous humor and vitina.4,the extent ofthe sVCAM-1 in aqueous humor and the VCAM-1 in retina decreased obviously treated by Aminoguanidine(75mg/kg) in early stage compared with control group,no further VCAM-1 decreased in 100mg/kg treatment group,the sVCAM-1 in aqueous humor and the VCAM-1 in retina have no any alternation in 50,25 mg/kg treatment group compare control group(P>0.05);5,there is no difference of the sVCAM-1 in aqueous humor and the VCAM-1 in retina between any dose treatment group and control group in adavanced DR stage,the pattern of sVCAM-1 in aqueous humor is same to the expression in retina.6,we found the correlation between the level of sVCAM-1 in aqueous humor and such phenomena : the aneurysms,pericyte ghosts,acellular capillary sidanohphilia and blood-retina barrier destroy treated by 75mg/kg and 100mg/kg but not 25mg/kg and 50mg/kg in early stage groups show significant difference with control group (P<0.05=, there is no difference in such indexes between all dose treatment group in advanced stage and control group.the destroy of rats ritina in early stage induced by the micro-morphologic change can be cured by 75mg/kg and other higher dose Aminoguanidine,the tendency is same with the effect on the level of sVCAM-1 treated by 75mg/kg Aminoguanidine. conclusion:one of the mechanism that diabetes induce the proliferation of retina is up-regulating the adhesion molecule , Aminoguanidine can suppress the extent of proliferation of retina in primary stage but not advanced stage induced by diabetes through suppress the adhesion molecule.it can prevent the formation of microangiopathy related to DR in retina.it can elucidate the adhesion molecule activated in primary stage of DR.
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