Objective To investigate the changes in the number and activity of endothelial progenitor cells(EPCs) from peripheral blood in patients with type 2 diabetes mellitus.Methods Choice type 2 diabetes mellitus patients (n=16) and control subjects(n=19), peripheral blood mononuclear cells(MNCs) were isolated by density gradient centrifugation. After 7 days induced differentiation, identified the adherent cells were EPCs by fiuorescein staining and flow cytometry. EPCs proliferation, adhesion and in vitro vasculogenesis activity were assayed by MTT chromatometry, adhesion activity assay essay and in vitro vasculogenesis kit respectively.Results The number of EPCs from peripheral blood weresignificantly reduced in patients with type 2 diabetes mellitus[ (3.1±1.2)×10~5]: [(3.9±1.1)±10~5], P < 0.05. In addition, the proliferation[( 0.170±0.056 ): (0.225±0.071) OD valve, P< 0.05], adhesion[ (50±15):( 60±11 ) cells/×200fields, P < 0.05] and in vitro vasculogenesis activity of EPCs in patients with type 2 diabetes mellitus were also impaired.Conclusion Endothelial Progenitor Cells from peripheral blood in patients with type 2 diabetes mellitus exhibit reduced number and impaired proliferation, adhesion and in vitro vasculogenesis activity.
|