Biological Modification With RGD Peptide Grafted On Titanium And Preparation Of HA Coating With Medicine

Titanium and its alloys have been applied in orthopedics more and more owing to its biocompatibility and bioinertness. As implant materials its surface characteristics such as surface chemistry and surface texture have a great effect on the integration of the material after surgery. Proteins of the extracellular matrix are known to be important for cell attachement and spreading on biomaterials. The main objective of this paper is to graft the peptide Arg-Gly-Asp (RGD) covalently onto the surface of pure titanium by self assembly method (SAM) to improve its bioactivity. Based on apatite precipitation, protein adsorption and osteoblast culture in vitro on the peptide modified surface, the surface characteristics were studied.Biomimetic modification was performed by means of self-assembly method to graft the peptides RGD on the titanium surface. The elements of C, N, O and Si were detected by X-ray Photoelectron Spectroscopy (XPS) analysis. And from the Fourier transformed infrared spectra (FTIR) the corresponding chemical groups were observed. The results indicated that the RGDC was grafted on the titanium surface covalently. The in vitro biomimetic mineralization was also investigated. On the peptide modified surface the apatite coating formed. It showed that the end groups on modified surface are beneficial to apatite deposition. The investigation on bovine serum albumin (BSA) adsorption indicated that BSA was adsorbed much more easily on the titanium surface containing peptides. The osteoblast culture test showed that the activity of cell on the peptide modified surface was higher than that on the pure titanium. It indicated that the peptide RGD to promote cell adhesion, spread and colonization. It was observed there was high cell activity on the apatite coating precipitated on the peptide modified surface. Additionally, Incorporation of antibiotics in the apatite coatings on titanium surface is an effective step for surgical infections of titanium implant. In this work, antibiotics cephradine and traditional Chinese medicine salviae miltlorrhizae (SM) were incorporated in the simulated body fluid (SBF). The apatite coatings were coprecipitated with cephradine and SM by a biomimetic method. The research showed that in the biomimetic coprecipitation process cephradine containing carboxylic groups was beneficial to the apatite coprecipitation. The morphology of the coating formed in SBF with cephradine was spherical compared with that formed in SBF. X-ray diffraction patterns showed that cephradine had a positive effect on the crystal growth in terms of cystallinity. And also in the FTER the organic groups corresponded with cephradine were also observed. However, little apatite formed on the titanium immersed in SBF with SM. Probably, SM was more absorbed on the surface when calcium and phosphate ions precipitated. The role of SM in the coprecipitation was still unclear since chemical components of SM were much more and complicated.