The Role Of Redox Factor 1 In Ischemia Preconditioning For Reduced-for-size Liver Transplantation Of Rats

Objective To Build the model of little animal liver transplantation is the basis of experimental research. Usually the liver transplantation model of rats is used, for example, classical Kamada model "double bushing method", etc. In order to investigate deeply the protective mechanism of ischemia preconditioning(IPC) on ischemia reperfusion injury of graft after reduced-for-size liver transplantation of rats, we establish stable reduced-for-size liver transplantation ( RSLT ) model of rats by referring to Lixiangcheng's methods and using improved "double bushing method". Objective To observe the effects of ischemia precondition on the expression of Ref-1 protein after reduced-size liver transplantation of rats and approach the protection mechanisms of IPC for liver injury. Methods 100 adult male Lewis rats were randomly divided into three groups: 50% liver transplantation (PLT group), 50% liver transplantation with ischemia preconditioning (IPC group) and sham operation group (SO group). Animals were sacrificed in each group at different time points: 0.5h,2h,6h and 24h after liver transplantation. Then, we explore the changes and significance of the expression of Ref- 1 according to westernblotting and immunohistochemistry, associated with serology and histopathology analysis. Results The serum ALT values of animal in PLT group for 0.5h,2h,6h and 24h after liver transplantation are (595.06±108.78)U/L; (723.18±117.24)U/L; (1186.65±142.31)U/L;(1498.91±126.79)U/L; and (459.06±84.73) U/L;(587.71±95.23)U/L;(799.61±125.97)U/L;(659.27±135.68)U/L for IPC group. As compared with PLT group, the ALT values at 6h and 24h after operation in IPC group decrease significantly (t=4.553,P＜0.05; t=10.110, P＜0.01). By the pathobiological analysis, we find there are lots of inflammation cells infiltration around the portal veins, the serious sinus hepaticus dilation and damage of hepatic tissue. However, the damage in IPC group are comparatively slight. Our results reveal the increased expression of Ref-1 protein in both IPC group and PLT group comparing with SO group. Of significance, the expression of Ref-1 protein in IPC group is higher than that in PLT group at 24h after reduced-size liver transplantation (p＜0.05). Conclusion These data suggest that ischemia preconditioning relieve the liver injury after reduced-size liver transplantation in earlier period, and facilitate the expression of Ref-1 protein in hepatic tissue after transplantation, which imply the protection mechanism of ischemia preconditioning to ischemia reperfusion injury of graft in early period after reduced-size liver transplantation are partly at least relevant with the promotion of Refol protein expression.