Isolation And Structure Identification Of The Chemical Constituents And Xanthine Oxidase Inhibiting Activity Of Amure

Objective: Amure is mainly distributed in the south area of Yunnan province. The root of the plant is used as a folk medicine for clearing away heat and toxic material, diuresis, treating of traumatic injury and arthritis. Its medicative for gout as a folk medicine has been watched in long clinical application. The studies on xanthine oxidase inhibiting activity of amure and its chemical constituents have not been reported. Studies on the chemical constituents of amure have been carried out for finding out the xanthine oxidase inhibiting constituents.Methods: Systematic solvents extraction has been made by Et₂O, CHCl₃, EtOAc, EtOH and water in succession. Column chromatography on silica gel and macro-reticular resine column have been carried out for the further separation and purification of those extracts of amure, 8 compounds have been re-crystallized. The structures of seven compounds of them were identified by classical chemical methods and spectroscopic methods analysis (MS, ¹HNMR, ¹³ CNMR, DEPT). The structure of another one compound need being further identified. The xanthine oxidase inhibiting activities of these compounds has been investigated.Results: 8 compounds were isolated. The structures of seven compounds of them were determined as 2, 4-sorbic acid(â… ), Spinasterol (â…¡) , Daucosterol (â…¦), Taraxerone(â…§). Compoundâ…£,â…¤,â…¦showed xanthine oxidase (XO) inhibiting activities with IC₅₀ value of 85．05μg/ml,97．50μg/ml,93．10μg/ml respectively in vitro.Conclusion: Eight compounds were isolated and separated from EtOH extracts of amure. Seven of thire chemical structures have been definited. Compoundâ…¥is a new natural product. Compoundâ…£showed xanthine oxidase (XO) inhibiting activity with IC₅₀ value of 85．05μg/ml.缩略语表缩略语英文中文TLC Thin layer chromatography薄层层析MP Melting point熔点NMR Nuclear magnetic resonance核磁共振谱DMSO Dimethyl sulloxide二甲基亚砜EtOAc Ethyl acetate乙酸乙酯EtOH Ethanol乙醇MeOH Methanol甲醇CHC13 Chloroform氯仿Distortionless enhancement byDEPT无畸变激化转移增益法polarization transfer¹H Detected Heteronuclear Multiple-hmqc ¹H检测的异核多量子相干相关谱Quantum Transfer Experiment ¹H Detected Heteronuclear Multiple Bondhmbc ¹H检测的异核多键相关谱ConectivityHeteronuclear Single-Quantum HSQC ¹H检测的异核单量子相关谱Coherence...