| Objective: To investigate the role ofα-MSH in acute lung injury (ALI) associated with severe acute pancreatitis (SAP).Material and Method: A total of thirty Sprague-Dawley rats were randomly divided into sham operation group, SAP group andα-MSH treatment group. The model of SAP was established by retrograde pancreatic injection of sodium taurocholate(1 ml/kg). The model of sham operation group was established by retrograde pancreatic injection of 0.9% sodium chloride. The model ofα-MSH treatment group was the same as SAP group an hour later by celiac injection ofα-MSH. Intrapulmonary expression of MIF was assayed by streptavidin peroxidase conjugated method(SP). Meanwhile, serum level of amylase, ascites volume, pulmonary and pancreatic wet/dry weight ratio and pulmonary and pancreatic histological grading were also measured.Results: 1.serum level of amlyse,pancreatic and pulmonary wet/dry weight ratio,the histological scores of pulmonary and pancrease,the expression of MIF in pulmonary in SAP groupwas very high. While in the sham operation group they were normal.2.After the treatment ofα-MSH ,serum level of amlyse,pancreatic and pulmonary wet/dry weight ratio were markly decreased;the histological scores of pulmonary and pancrease and the expression of MIF in pulmonary were decreased,with statistical significance compared with the SAP group(P<0.01). 3.the expression of MIF in pulmonary had positive correlation with the score of lunginjury.r=0.579Conclusion: The treatment ofα-MSH showed lung and pancreas protective eftect.Themechanism may be by the inhibitory action ofα-MSH on the expression of MIF thatcan inhibite the release of cytokine. Meanwhile ,it can reduce severity of SAP. |
