| Objective To investigate the radiosensitization of a Oridonin in Hepatic cancer cell linesin vitro. Methods Clonogenic assay was used to investigate the radiosensitizing effect ofOridonin on a human hepatic cancer cell line HepG2 cancer cell lines. After Treat withoridonin of 1.804, 3.608, 5.412, 7.216 and 18.04μmol/L for 6～72 hours, Give the HepG2cell clinically relevant dose of 0, 1, 2, 3.4, 5, 6 Gy. Observe the survival fraction (SF).calculate the sensitization enhancement ratio (SER). change in radiosensitivity wasquantified by calculating the sensitization enhancement ratio at a clinically relevant doseof 2Gy (SERSF2). Results Oridonin enhanced the radiosensitivity of HepG2 cells in atime-course dependent manner, and the radiosensitizing effect was dependent on thedoses of Oridonin. After treatment with 1.804μmol/L Oridonin for 6～72h. noradiosensitization was observed at 6, 12 and 24h. whereas an enhancement ofradiosensitivity was seen at 48h and 72h. With a high dose of 18.040μmol/L Oridoninexposed to HepG2 cells for 6, 12, and 24h, the enhancement of radiosensitivi y was seen.Conclusion These results show that Oridonin is a botential radiosensitizer in llepaticcancer cell lines.
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