Comparison Proteomic Study On HBV Related Chronic Hepatitis, Liver Cirrhosis And Primary Hepatic Carcinoma

Objective: In this study, we tried to detect and identify the special protein of HBV related chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. To find new opinion on the developing of chronic liver disease. Methods: The sera of health adult, HBV related chronic hepatitis, liver cirrhosis and hepatocellular carcinoma were respectively detected by surface enhanced laser desorption/ionization time-of- flight mass spectrometry (SELDI-TOF-MS). The arrays of every group were analysised by clustering analysis and to establish disease predictive model. Then the sample was eluted with different pH Tris, trypsinization on-chip, mass determination and peptide database comparison. Results:â‘ According CM10 chip we find 18 protein with obviously deviation (P<0.05) among HBV related chronic hepatitis, liver cirrhosis and hepatocellular carcinoma.â‘¡Clustering analysis for the data from SELDI-TOF-MS confirmed 42 differentially expressed proteins. Then we developed disease predictive mathematic models ( Decision Tree Model, DT model ) with average validity up to 73.9%.â‘¢The 5805 Da protein peak was identified to be Chondroitin sulfate synthase 2 (CHSS2), which is a potential molecule involved in the pathologic process and a potential serum marker for the HBV related hepatic diseases as well. Conclusions: Our results suggest that SELDI-TOF-MS is a usefull technique for differential expressed proteins screening and analysis in HBV related chronic liver disease. CHSS2 may be useful during the developing of HBV related chronic liver disease.